Due to their technical features, the columns are versatile and usable for almost all separation problems in the biochemical research laboratory as well as in the pilot plant or in the production area. The column design allows the separation of gram quantities of a HPLC with analog resolution. This feature is guaranteed by the ideal flow characteristics of the column adapter.
THOMACHROM®-MPLC-columns are indispensable components for progressive liquid chromatography.
Max. pressure load up to 20 bar
Sample application without dead-volume
Resistant to organic solvents
Separation of gram-quantities with high resolution capability
The column body is made of borosilicate glass with smooth inner surface. The shaping of the end of the glass body guarantees high efficiency due to even zone travel. The symmetrical design of THOMACHROM®-MPLC-column prevents preferred flow directions of the eluent. Therefore, no "dead zones" exist. Column end pieces (column adapters) plug the column on both sides. The column adapters and the adapter frites (25 µm) are made of PTFE or glass sintered plates. The connecting capillary tube (outside diameter 1/16") is made of chromate (material 4571).
The column ends are conically shaped and fitted with a screw thread.
The column adapter is introduced in the column end, the sealing between adapter and column inner surface being guaranteed by four lip packings. For arresting the column adapter a cap screw is then screwed on each end of the column, pressing the adapter firmly into the column body, thus securing the seal.
The design of the adapter guarantees an even sample distribution and consequently reproducible values. As the connecting capillary tube rests planely on the adapter frit, no dead volume arises in the adapter. The sample is directly taken up by the adapter frit and atomized.
Several THOMACHROM®-MPLC-columns are easily to be coupled together. "Paralleling" is used for preparative purposes, i.e. for separation of large substance quantities. "Cascade connection" is used for increasing the number of plates, i.e. for improvement of the resolution.
A series of chromatography columns in eight different sizes for analytical and preparative purposes. The glass bodies are made of borosilicate glass, flange-connected on both sides, and bear fixedly two caps with the sealing O-rings belonging to it. Caps with the largest diameters of 75 and 100 mm resp. have threaded bolts made of V2A and are screwed with nuts to the end plates. Caps and end plates of smaller sizes are screwed together with threads. In this case the caps bear the sealing rings of the cooling jacket. The collars of the cooling jacket fit tightly on the sealing rings of the cooling jacket. Is no cooling jacket mounted, the sealing rings serve as shock absorbers to avoid break when the column is put down on the laboratory desk. Diameters of 75 mm and 100 mm resp. are not supplied with cooling jacket. For temperature control the use of Calorex heat-exchange and cooling tubing (item 93168) is recommended.
The bottom end plates bear a PTFE fabric with a mesh size of approx. 10 µm as support for the adsorbent material. The fabric is fixed by means of a locking ring and is placed on a PTFE grid of larger mesh size. In this grid the fluid flows with minimum dead volume to the capillary bore of the end plate.
The column end plates have thread bores for accommodation of MULTICHROM connections, the upper end plate having an additional one for the bleed screw. The central bore has an internal diameter of 1.0 mm.
The fluid contacting parts of the column, the top and bottom ends, are usually made of PVC. For use with liquids, to which these materials are not resistant, ends made of PTFE are to be used. Therefore, only these two exchange parts are required for resetting of the column, and already with the usual caps made of PVC a substantial solvent resistance is obtained. When working for a longer time with media particularly aggressive to PVC, such as chloroform, dimethylformamide etc, the glass bodies with caps made of stainless steel, PTFE or PP have to be used.
We strongly emphasize that standard seals are made of the materials FPM and NBR. It is possible to substitute certain seals for those made the material FFKM. The THOMACHROM® system with its many combinations offers appropriate instrumental solutions for pressures up to 15 bar and for all solvents, particularly for preparative purposes.
Inside Ø column