{"id":14282,"date":"2019-04-18T15:38:54","date_gmt":"2019-04-18T13:38:54","guid":{"rendered":"https:\/\/www.rct-online.de\/magazin\/?p=14282"},"modified":"2025-07-11T16:11:06","modified_gmt":"2025-07-11T14:11:06","slug":"hplc-a-method-for-biochemical-research","status":"publish","type":"post","link":"https:\/\/www.rct-online.de\/magazin\/en\/hplc-a-method-for-biochemical-research\/","title":{"rendered":"HPLC \u2013 A Method for Biochemical Research"},"content":{"rendered":"<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_73 ez-toc-wrap-center counter-hierarchy ez-toc-counter ez-toc-white ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title\" style=\"cursor:inherit\">Table of contents<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #999;color:#999\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #999;color:#999\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/www.rct-online.de\/magazin\/en\/hplc-a-method-for-biochemical-research\/#What_Is_Liquid_Chromatography\" title=\"What Is Liquid Chromatography?\">What Is Liquid Chromatography?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/www.rct-online.de\/magazin\/en\/hplc-a-method-for-biochemical-research\/#How_Does_HPLC_Work\" title=\"How Does HPLC Work?\">How Does HPLC Work?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/www.rct-online.de\/magazin\/en\/hplc-a-method-for-biochemical-research\/#Applications_of_High-Performance_Liquid_Chromatography\" title=\"Applications of High-Performance Liquid Chromatography\">Applications of High-Performance Liquid Chromatography<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/www.rct-online.de\/magazin\/en\/hplc-a-method-for-biochemical-research\/#Relevance_in_Biochemistry\" title=\"Relevance in Biochemistry\">Relevance in Biochemistry<\/a><\/li><\/ul><\/nav><\/div>\n<p style=\"text-align: justify;\"><strong>HPLC stands for High Performance Liquid Chromatography. This technique is used for both preparative and analytical separations. In biochemical research, HPLC is especially useful for preparing and analyzing substances with high molecular weight, such as proteins.<\/strong><\/p>\n<h2><span class=\"ez-toc-section\" id=\"What_Is_Liquid_Chromatography\"><\/span>What Is Liquid Chromatography?<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p style=\"text-align: justify;\">Chromatography is a separation technique in which a mixture is divided between two phases: a stationary phase and a mobile phase. In liquid chromatography, the stationary phase is a solid, while the mobile phase is a liquid, commonly referred to as the <em>eluent<\/em>.<\/p>\n<blockquote><p>As the mobile phase flows past the stationary phase, substances within the mixture interact differently with the surface of the stationary material. These varying interactions lead to different flow velocities and ultimately to separation.<\/p><\/blockquote>\n<p style=\"text-align: justify;\">Various materials can serve as the stationary phase: for instance, special filter papers in paper chromatography, or silica gel and aluminum oxide in column and thin-layer chromatography. Mobile phases range from non-polar solvents like hexane to polar solvents such as water.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"How_Does_HPLC_Work\"><\/span>How Does HPLC Work?<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p style=\"text-align: justify;\">HPLC is essentially an advanced form of column chromatography. In this method, a high-pressure pump (typically 50\u2013400 bar) pushes the mobile phase through a column filled with the stationary phase, ensuring smooth and pulse-free flow.<\/p>\n<p style=\"text-align: justify;\">Once a sample is introduced via an injector at the start of the column, separation occurs inside the column. A downstream detector records a signal proportional to the amount of each component eluted and presents this data in a graphical format known as a chromatogram. Ideally, each peak in the chromatogram corresponds to a distinct component of the mixture.<\/p>\n<figure id=\"attachment_3522\" aria-describedby=\"caption-attachment-3522\" style=\"width: 600px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-3522 size-full\" title=\"HPLC-Pump\" src=\"https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/hplc-pumpe.jpg\" alt=\"HPLC-Pump - HPLC \u2013 A Method for Biochemical Research\" width=\"600\" height=\"405\" srcset=\"https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/hplc-pumpe.jpg 600w, https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/hplc-pumpe-300x203.jpg 300w, https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/hplc-pumpe-110x75.jpg 110w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><figcaption id=\"caption-attachment-3522\" class=\"wp-caption-text\">\u00a9 Ioana Davies (Drutu) \u2013 stock.adobe.com<\/figcaption><\/figure>\n<p>Commonly used detectors include UV-absorbance, refractive index, and fluorescence detectors. Recycling uncontaminated eluents helps reduce solvent consumption significantly.<\/p>\n<p style=\"text-align: justify;\">Compared to traditional gravity-driven column chromatography, HPLC works with smaller sample volumes, much faster elution rates, and considerably higher resolution. Heating the column via a column oven can alter interactions between mobile and stationary phases, thus influencing separation performance.<\/p>\n<figure id=\"attachment_3533\" aria-describedby=\"caption-attachment-3533\" style=\"width: 600px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-3533 size-full\" title=\"HPLC System Agilent 1200\" src=\"https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/HPLC-System-Agilent-1200.jpg\" alt=\"HPLC System Agilent 1200\" width=\"600\" height=\"800\" srcset=\"https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/HPLC-System-Agilent-1200.jpg 600w, https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/HPLC-System-Agilent-1200-225x300.jpg 225w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><figcaption id=\"caption-attachment-3533\" class=\"wp-caption-text\">The modern HPLC system Agilent 1200 | \u00a9 Kommando \u2013 en.wikipedia.org<\/figcaption><\/figure>\n<p>Ultra-high-performance liquid chromatography (UHPLC) takes things a step further by using even smaller particles and shorter, narrower columns\u2014further increasing resolution.<\/p>\n<p style=\"text-align: justify;\">The following section provides a brief overview of the key components in a high-performance liquid chromatography system:<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-14300 size-full\" title=\"HPLC ELuent\" src=\"https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/04\/hplc-eluent-probe-6.jpg\" alt=\"HPLC ELuent\" width=\"600\" height=\"294\" srcset=\"https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/04\/hplc-eluent-probe-6.jpg 600w, https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/04\/hplc-eluent-probe-6-300x147.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/p>\n<ul style=\"text-align: justify;\">\n<li>Sample Injection<\/li>\n<\/ul>\n<p style=\"text-align: justify;\">Samples are typically injected using fixed-volume sample loops. A six-port valve switches the flow path so that the eluent pushes the sample from the loop onto the column.<\/p>\n<p style=\"text-align: justify;\">At low to medium pressure, manual injection using syringes is possible. In automated settings, autosamplers minimize manual workload. The injected volume depends on the column&#8217;s diameter and typically ranges from 1\u202f\u00b5L to 2\u202fmL.<\/p>\n<figure id=\"attachment_3527\" aria-describedby=\"caption-attachment-3527\" style=\"width: 600px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-3527 size-full\" title=\"Autosampler in Chromatography\" src=\"https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/autosampler-chromatographie.jpg\" alt=\"Autosampler in Chromatography - HPLC \u2013 A Method for Biochemical Research\" width=\"600\" height=\"402\" srcset=\"https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/autosampler-chromatographie.jpg 600w, https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/autosampler-chromatographie-300x201.jpg 300w, https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/autosampler-chromatographie-110x75.jpg 110w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><figcaption id=\"caption-attachment-3527\" class=\"wp-caption-text\">\u00a9 Yen-yu Shih \u2013 stock.adobe.com<\/figcaption><\/figure>\n<ul>\n<li style=\"text-align: justify;\">Chromatography Column<\/li>\n<\/ul>\n<p>Since pressures up to 400 bar are applied, columns are made from thick-walled glass, often sheathed in pressure-resistant stainless steel for safety. However, stainless steel can interact with bioactive substances, making it unsuitable for many biochemical applications. For this reason, bioinert materials such as titanium are sometimes used. Internal diameters usually measure only a few millimeters.<\/p>\n<p><center><a href=\"https:\/\/www.rct-online.de\/en\/tubes-hoses\/tubings-and-pipes-made-of-rigid-plastics\/peek-tubing\/28755\/peek-high-pressure-capillary-tube-with-stripes\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-3537 size-full\" title=\"PEEK High-Pressure Capillary Tube | with stripes\" src=\"https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/peek-hochdruck-kapillarschlauch-gestreift.jpg\" alt=\"PEEK High-Pressure Capillary Tube | with stripes\" width=\"300\" height=\"300\" srcset=\"https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/peek-hochdruck-kapillarschlauch-gestreift.jpg 300w, https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/peek-hochdruck-kapillarschlauch-gestreift-150x150.jpg 150w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/a> <a href=\"https:\/\/www.rct-online.de\/en\/tubes-hoses\/tubings-and-pipes-made-of-rigid-plastics\/peek-tubing\/28754\/peek-high-pressure-capillary-tube-solid-colour\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-3538 size-full\" title=\"PEEK High-Pressure Capillary Tube | solid colour\" src=\"https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/peek-hochdruck-kapillarschlauch-unifarbig.jpg\" alt=\"PEEK High-Pressure Capillary Tube | solid colour\" width=\"300\" height=\"300\" srcset=\"https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/peek-hochdruck-kapillarschlauch-unifarbig.jpg 300w, https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/peek-hochdruck-kapillarschlauch-unifarbig-150x150.jpg 150w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/a><\/center><\/p>\n<ul style=\"text-align: justify;\">\n<li>Stationary Phase<\/li>\n<\/ul>\n<p style=\"text-align: justify;\">HPLC columns are densely packed with solid, porous, and pressure-stable materials\u2014typically functionalized silica gels. In contrast to low-pressure chromatography, the particle size in HPLC is significantly smaller, usually &lt;\u202f10\u202f\u03bcm, which improves resolution.<\/p>\n<p style=\"text-align: justify;\">Separation may be based on adsorption, ion exchange, or size exclusion, allowing for a wide range of applications.<\/p>\n<p><center><a href=\"https:\/\/www.rct-online.de\/en\/tube-hose-connectors\/connectors-made-of-plastic\/capillary-connectors-for-hplc\/28871\/straight-capillary-connector-with-male-thread-made-of-pp\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-3549 size-full\" title=\"Straight Capillary Connector with Male Thread made of PP\" src=\"https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/gerader-kapillar-verbinder-mit-aussengewinde-aus-pp.jpg\" alt=\"Straight Capillary Connector with Male Thread made of PP\" width=\"300\" height=\"300\" srcset=\"https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/gerader-kapillar-verbinder-mit-aussengewinde-aus-pp.jpg 300w, https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/gerader-kapillar-verbinder-mit-aussengewinde-aus-pp-150x150.jpg 150w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/a> <a href=\"https:\/\/www.rct-online.de\/en\/tube-hose-connectors\/connectors-made-of-plastic\/capillary-connectors-for-hplc\/28886\/high-pressure-straight-capillary-connector-made-of-peek\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-3550 size-full\" title=\"High-Pressure Straight Capillary Connector made of PEEK\" src=\"https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/hochdruck-gerader-kapillar-verbinder-aus-peek.jpg\" alt=\"High-Pressure Straight Capillary Connector made of PEEK\" width=\"300\" height=\"300\" srcset=\"https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/hochdruck-gerader-kapillar-verbinder-aus-peek.jpg 300w, https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/hochdruck-gerader-kapillar-verbinder-aus-peek-150x150.jpg 150w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/a><\/center>Most often, separation is based on differences in polarity between the mobile and stationary phases. A distinction is made between normal-phase HPLC (NP), which uses a polar stationary phase and a non-polar mobile phase, and reverse-phase HPLC (RP), where the polarities are reversed.<\/p>\n<p style=\"text-align: justify;\">The polarity of both phases strongly influences retention time\u2014the time a substance takes to travel through the column. A substance with weak interactions with a non-polar stationary phase has a shorter retention time.<\/p>\n<p style=\"text-align: justify;\">Although normal-phase HPLC can be difficult to manage (e.g. due to water contamination in solvents), reverse-phase HPLC is far more commonly used in practice.<\/p>\n<p><center><a href=\"https:\/\/www.rct-online.de\/en\/tube-hose-connectors\/connectors-made-of-metal\/capillary-connectors-for-hplc\/29235\/straight-capillary-coupling-made-of-stainless-steel\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-3554 size-full\" title=\"Straight Capillary Coupling made of Stainless Steel | complete\" src=\"https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/gerader-kapillarverbinder-aus-edelstahl-komplett.jpg\" alt=\"Straight Capillary Coupling made of Stainless Steel | complete\" width=\"300\" height=\"150\" \/><\/a> <a href=\"https:\/\/www.rct-online.de\/en\/tube-hose-connectors\/connectors-made-of-metal\/capillary-connectors-for-hplc\/29243\/male-screw-made-of-stainless-steel-1.4571\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-3555 size-full\" title=\"Male Screw made of Stainless Steel 1.4571\" src=\"https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/hohlschraube-aus-edelstahl-1-4571.jpg\" alt=\"Male Screw made of Stainless Steel 1.4571\" width=\"300\" height=\"300\" srcset=\"https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/hohlschraube-aus-edelstahl-1-4571.jpg 300w, https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/hohlschraube-aus-edelstahl-1-4571-150x150.jpg 150w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/a><\/center>&nbsp;<\/p>\n<ul style=\"text-align: justify;\">\n<li>Mobile Phase<\/li>\n<\/ul>\n<p style=\"text-align: justify;\">Polarity charts and elutropic series can help select appropriate eluents. Mixtures of solvents are often used to fine-tune polarity and elution strength. Electronic mixers allow for dynamic gradient programming of solvent composition.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Applications_of_High-Performance_Liquid_Chromatography\"><\/span>Applications of High-Performance Liquid Chromatography<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<ul>\n<li style=\"text-align: justify;\">Analytical Use<\/li>\n<\/ul>\n<p style=\"text-align: justify;\">Identification is typically done by comparing a sample\u2019s retention time with that of a reference substance. Mixing the sample with the reference can confirm identity: a single, larger peak suggests a match; two peaks indicate non-identity. Repeating the process with a different mobile phase helps rule out accidental peak overlaps.<\/p>\n<p style=\"text-align: justify;\">Even greater certainty is achieved by switching the stationary phase (e.g., from NP- to RP-HPLC). However, full confirmation requires qualitative identification of the eluted substance. This can be accomplished using specialized detectors such as mass spectrometry or diode-array systems that record entire UV\/Vis spectra.<\/p>\n<ul style=\"text-align: justify;\">\n<li>Preparative Use<\/li>\n<\/ul>\n<p style=\"text-align: justify;\">HPLC is also used for purification\u2014just like classical column chromatography. This is common in pharmaceutical or biological sample preparation. Larger internal diameters are used to accommodate the greater volumes typical of preparative applications.<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Relevance_in_Biochemistry\"><\/span>Relevance in Biochemistry<span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p style=\"text-align: justify;\">HPLC\u2019s versatility stems from its wide array of separation modes and detection methods. In biochemical laboratories, it\u2019s indispensable for analyzing complex mixtures of biopolymers\u2014such as nucleic acids, amino acids, peptides, or enzymes. With proper scaling, HPLC can also purify target compounds from complex matrices. It is therefore a standard tool in modern biochemical research.<\/p>\n<blockquote>\n<p style=\"text-align: justify;\">HPLC is also used in medical diagnostics, for example, in determining vitamin D levels in blood samples.<\/p>\n<\/blockquote>\n<p style=\"text-align: justify;\">Other typical applications include polymer analysis, detection of active ingredients and contaminants, purity testing, and quantitative analysis of substances in biological samples. HPLC is also commonly used in drinking water testing. HPLC is routinely employed in environmental monitoring and water quality testing across Europe.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>HPLC stands for High Performance Liquid Chromatography. This technique is used for both preparative and analytical separations. In biochemical research, HPLC is especially useful for preparing and analyzing substances with high molecular weight, such as proteins. What Is Liquid Chromatography? Chromatography is a separation technique in which a mixture is divided between two phases: a &hellip;<\/p>\n","protected":false},"author":7,"featured_media":11863,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1,2744],"tags":[3102,3101,3110,3113,3112,3111,3103,3115,3107,3108,3109,3116,3105,3117,2774,3106,3104,3114],"class_list":["post-14282","post","type-post","status-publish","format-standard","has-post-thumbnail","","category-all-articles","category-chemical-technology-in-action","tag-biochemical","tag-biochemistry","tag-capillary","tag-capillary-connector","tag-capillary-hose","tag-capillary-tube","tag-chromatography","tag-connector","tag-high-pressure","tag-high-performance-liquid-chromatography","tag-hollow-screw","tag-hplc-en","tag-liquid-chromatography","tag-peek-en","tag-pp-en","tag-research","tag-stainless-steel","tag-titanium"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.1 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>HPLC \u2013 A Method for Biochemical Research - Reichelt Chemietechnik Magazine<\/title>\n<meta name=\"description\" content=\"HPLC (High Performance Liquid Chromatography) for preparative and analytical substance separation: operating principle and application examples. Learn more in the RCT Online Magazine!\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.rct-online.de\/magazin\/en\/hplc-a-method-for-biochemical-research\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"HPLC \u2013 A Method for Biochemical Research - Reichelt Chemietechnik Magazine\" \/>\n<meta property=\"og:description\" content=\"HPLC (High Performance Liquid Chromatography) for preparative and analytical substance separation: operating principle and application examples. Learn more in the RCT Online Magazine!\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.rct-online.de\/magazin\/en\/hplc-a-method-for-biochemical-research\/\" \/>\n<meta property=\"og:site_name\" content=\"Reichelt Chemietechnik Magazine\" \/>\n<meta property=\"article:published_time\" content=\"2019-04-18T13:38:54+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2025-07-11T14:11:06+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/hplc.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"800\" \/>\n\t<meta property=\"og:image:height\" content=\"480\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Dr. Karl-Heinz Heise\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Dr. Karl-Heinz Heise\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"8 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.rct-online.de\/magazin\/en\/hplc-a-method-for-biochemical-research\/\",\"url\":\"https:\/\/www.rct-online.de\/magazin\/en\/hplc-a-method-for-biochemical-research\/\",\"name\":\"HPLC \u2013 A Method for Biochemical Research - Reichelt Chemietechnik Magazine\",\"isPartOf\":{\"@id\":\"https:\/\/www.rct-online.de\/magazin\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/www.rct-online.de\/magazin\/en\/hplc-a-method-for-biochemical-research\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/www.rct-online.de\/magazin\/en\/hplc-a-method-for-biochemical-research\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/hplc.jpg\",\"datePublished\":\"2019-04-18T13:38:54+00:00\",\"dateModified\":\"2025-07-11T14:11:06+00:00\",\"author\":{\"@id\":\"https:\/\/www.rct-online.de\/magazin\/#\/schema\/person\/de5369aedc25d1f064871d823629e056\"},\"description\":\"HPLC (High Performance Liquid Chromatography) for preparative and analytical substance separation: operating principle and application examples. Learn more in the RCT Online Magazine!\",\"breadcrumb\":{\"@id\":\"https:\/\/www.rct-online.de\/magazin\/en\/hplc-a-method-for-biochemical-research\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.rct-online.de\/magazin\/en\/hplc-a-method-for-biochemical-research\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.rct-online.de\/magazin\/en\/hplc-a-method-for-biochemical-research\/#primaryimage\",\"url\":\"https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/hplc.jpg\",\"contentUrl\":\"https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/hplc.jpg\",\"width\":800,\"height\":480,\"caption\":\"\u00a9 Sirer \u2013 stock.adobe.com\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.rct-online.de\/magazin\/en\/hplc-a-method-for-biochemical-research\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/www.rct-online.de\/magazin\/en\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"HPLC \u2013 A Method for Biochemical Research\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.rct-online.de\/magazin\/#website\",\"url\":\"https:\/\/www.rct-online.de\/magazin\/\",\"name\":\"Reichelt Chemietechnik Magazine\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.rct-online.de\/magazin\/?s={search_term_string}\"},\"query-input\":\"required name=search_term_string\"}],\"inLanguage\":\"en-US\"},{\"@type\":\"Person\",\"@id\":\"https:\/\/www.rct-online.de\/magazin\/#\/schema\/person\/de5369aedc25d1f064871d823629e056\",\"name\":\"Dr. Karl-Heinz Heise\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\/\/www.rct-online.de\/magazin\/#\/schema\/person\/image\/\",\"url\":\"https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2024\/07\/KH_Heise.jpg\",\"contentUrl\":\"https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2024\/07\/KH_Heise.jpg\",\"caption\":\"Dr. Karl-Heinz Heise\"},\"description\":\"Dr. Karl-Heinz Heise studierte an der Martin-Luther Universit\u00e4t Halle-Wittenberg Chemie und der vormaligen Technischen Hochschule Dresden Radiochemie und Chemische Kerntechnik. Danach war er bis zur politischen Wende 1989 als wissenschaftlicher Mitarbeiter am Zentralinstitut f\u00fcr Kernforschung Rossendorf (ZfK) der Akademie der Wissenschaften in verschiedenen Bereichen der Isotopenproduktion und Markierungschemie t\u00e4tig. 1990 wurde er im neu gegr\u00fcndeten Leibnitz-Forschungszentrum Dresden - Rossendorf, dem heutigen Helmholtz-Zentrum, mit der Leitung der Abteilung f\u00fcr Organische Tracerchemie des Instituts f\u00fcr Radiochemie betraut, die sich mit umweltchemischen Prozessen in den Hinterlassenschaften des Uranbergbaus der DDR befasste. Dr. Heise ist begeisterter Hobby-Numismatiker und besch\u00e4ftigt sich dabei vornehmlich mit der h\u00f6fischen Medaillenkunst des 19. Jahrhunderts in Sachsen.\",\"url\":\"https:\/\/www.rct-online.de\/magazin\/en\/author\/k-heise\/\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"HPLC \u2013 A Method for Biochemical Research - Reichelt Chemietechnik Magazine","description":"HPLC (High Performance Liquid Chromatography) for preparative and analytical substance separation: operating principle and application examples. Learn more in the RCT Online Magazine!","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.rct-online.de\/magazin\/en\/hplc-a-method-for-biochemical-research\/","og_locale":"en_US","og_type":"article","og_title":"HPLC \u2013 A Method for Biochemical Research - Reichelt Chemietechnik Magazine","og_description":"HPLC (High Performance Liquid Chromatography) for preparative and analytical substance separation: operating principle and application examples. Learn more in the RCT Online Magazine!","og_url":"https:\/\/www.rct-online.de\/magazin\/en\/hplc-a-method-for-biochemical-research\/","og_site_name":"Reichelt Chemietechnik Magazine","article_published_time":"2019-04-18T13:38:54+00:00","article_modified_time":"2025-07-11T14:11:06+00:00","og_image":[{"width":800,"height":480,"url":"https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/hplc.jpg","type":"image\/jpeg"}],"author":"Dr. Karl-Heinz Heise","twitter_card":"summary_large_image","twitter_misc":{"Written by":"Dr. Karl-Heinz Heise","Est. reading time":"8 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/www.rct-online.de\/magazin\/en\/hplc-a-method-for-biochemical-research\/","url":"https:\/\/www.rct-online.de\/magazin\/en\/hplc-a-method-for-biochemical-research\/","name":"HPLC \u2013 A Method for Biochemical Research - Reichelt Chemietechnik Magazine","isPartOf":{"@id":"https:\/\/www.rct-online.de\/magazin\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.rct-online.de\/magazin\/en\/hplc-a-method-for-biochemical-research\/#primaryimage"},"image":{"@id":"https:\/\/www.rct-online.de\/magazin\/en\/hplc-a-method-for-biochemical-research\/#primaryimage"},"thumbnailUrl":"https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/hplc.jpg","datePublished":"2019-04-18T13:38:54+00:00","dateModified":"2025-07-11T14:11:06+00:00","author":{"@id":"https:\/\/www.rct-online.de\/magazin\/#\/schema\/person\/de5369aedc25d1f064871d823629e056"},"description":"HPLC (High Performance Liquid Chromatography) for preparative and analytical substance separation: operating principle and application examples. Learn more in the RCT Online Magazine!","breadcrumb":{"@id":"https:\/\/www.rct-online.de\/magazin\/en\/hplc-a-method-for-biochemical-research\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.rct-online.de\/magazin\/en\/hplc-a-method-for-biochemical-research\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.rct-online.de\/magazin\/en\/hplc-a-method-for-biochemical-research\/#primaryimage","url":"https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/hplc.jpg","contentUrl":"https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2019\/05\/hplc.jpg","width":800,"height":480,"caption":"\u00a9 Sirer \u2013 stock.adobe.com"},{"@type":"BreadcrumbList","@id":"https:\/\/www.rct-online.de\/magazin\/en\/hplc-a-method-for-biochemical-research\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/www.rct-online.de\/magazin\/en\/"},{"@type":"ListItem","position":2,"name":"HPLC \u2013 A Method for Biochemical Research"}]},{"@type":"WebSite","@id":"https:\/\/www.rct-online.de\/magazin\/#website","url":"https:\/\/www.rct-online.de\/magazin\/","name":"Reichelt Chemietechnik Magazine","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.rct-online.de\/magazin\/?s={search_term_string}"},"query-input":"required name=search_term_string"}],"inLanguage":"en-US"},{"@type":"Person","@id":"https:\/\/www.rct-online.de\/magazin\/#\/schema\/person\/de5369aedc25d1f064871d823629e056","name":"Dr. Karl-Heinz Heise","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.rct-online.de\/magazin\/#\/schema\/person\/image\/","url":"https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2024\/07\/KH_Heise.jpg","contentUrl":"https:\/\/www.rct-online.de\/magazin\/wp-content\/uploads\/2024\/07\/KH_Heise.jpg","caption":"Dr. Karl-Heinz Heise"},"description":"Dr. Karl-Heinz Heise studierte an der Martin-Luther Universit\u00e4t Halle-Wittenberg Chemie und der vormaligen Technischen Hochschule Dresden Radiochemie und Chemische Kerntechnik. Danach war er bis zur politischen Wende 1989 als wissenschaftlicher Mitarbeiter am Zentralinstitut f\u00fcr Kernforschung Rossendorf (ZfK) der Akademie der Wissenschaften in verschiedenen Bereichen der Isotopenproduktion und Markierungschemie t\u00e4tig. 1990 wurde er im neu gegr\u00fcndeten Leibnitz-Forschungszentrum Dresden - Rossendorf, dem heutigen Helmholtz-Zentrum, mit der Leitung der Abteilung f\u00fcr Organische Tracerchemie des Instituts f\u00fcr Radiochemie betraut, die sich mit umweltchemischen Prozessen in den Hinterlassenschaften des Uranbergbaus der DDR befasste. Dr. Heise ist begeisterter Hobby-Numismatiker und besch\u00e4ftigt sich dabei vornehmlich mit der h\u00f6fischen Medaillenkunst des 19. Jahrhunderts in Sachsen.","url":"https:\/\/www.rct-online.de\/magazin\/en\/author\/k-heise\/"}]}},"_links":{"self":[{"href":"https:\/\/www.rct-online.de\/magazin\/en\/wp-json\/wp\/v2\/posts\/14282"}],"collection":[{"href":"https:\/\/www.rct-online.de\/magazin\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.rct-online.de\/magazin\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.rct-online.de\/magazin\/en\/wp-json\/wp\/v2\/users\/7"}],"replies":[{"embeddable":true,"href":"https:\/\/www.rct-online.de\/magazin\/en\/wp-json\/wp\/v2\/comments?post=14282"}],"version-history":[{"count":18,"href":"https:\/\/www.rct-online.de\/magazin\/en\/wp-json\/wp\/v2\/posts\/14282\/revisions"}],"predecessor-version":[{"id":14390,"href":"https:\/\/www.rct-online.de\/magazin\/en\/wp-json\/wp\/v2\/posts\/14282\/revisions\/14390"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.rct-online.de\/magazin\/en\/wp-json\/wp\/v2\/media\/11863"}],"wp:attachment":[{"href":"https:\/\/www.rct-online.de\/magazin\/en\/wp-json\/wp\/v2\/media?parent=14282"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.rct-online.de\/magazin\/en\/wp-json\/wp\/v2\/categories?post=14282"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.rct-online.de\/magazin\/en\/wp-json\/wp\/v2\/tags?post=14282"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}